What should be noted in microbiological testing Microbial inspection notice there are a lot of details of aseptic operation, daily microbiological process should pay attention to aseptic operation, in addition to the clean bench and operating vessel on disinfection, also need to pay attention to health and environmental health. When training, the movements should be accurate and agile, but not too fast, to prevent air flow and increase the chances of pollution. Do not touch disinfected utensils by hand. If in contact, use flame to disinfect or replace with spare parts. For take convenient, the things on workbench face should have reasonable layout, should be in principle the thing that right hand USES is put in right, left hand thing is in left, alcohol lamp is placed in center. Work should be done in a certain order throughout, and tissue or cells should not be exposed to the air before treatment. Likewise, the culture medium should not be opened too early before it is used. If the bottle is not reused after use, it should be closed immediately. When absorbing nutrient solution, PBS, cell suspension and other kinds of liquid, all of them should use straws separately and not be mixed, in order to prevent the expansion of contamination or the cross-contamination of cells. Do not speak or cough in front of the operating field in order to prevent the saliva from bringing bacteria or mycoplasma into the working table. Use alcohol to wipe the surface and hands before operation. Hand or relatively dirty items should not pass over the top of the open bottle mouth, which is the most vulnerable to contamination. If the straw tip touches the bottle mouth when adding liquid, the straw should be discarded.
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